recombinant mouse ldlr associated protein  (R&D Systems)

Journal: Journal of lipid research

Article Title: Dennd5b-Deficient Mice are Resistant to PCSK9-Induced Hypercholesterolemia and Diet-Induced Hepatic Steatosis.

doi: 10.1016/j.jlr.2022.100296

Figure Lengend Snippet: Fig. 2. Dennd5b−/−affects plasma PCSK9 concentrations and hepatic LDLR abundance when fed a Western diet. A: PCSK9 was measured by ELISA in wild type and Dennd5b−/−mouse plasma collected on week 12. B: Hepatic LDLR protein was determined by Western blot on lysates from snap frozen tissue harvested on week 12. Statistical comparisons were performed by two-way ANOVA with Tukey correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001. LDLR, low density lipoprotein receptor; PCSK9, proprotein convertase subtilisin/kexin type 9.

Article Snippet: Lysates were used for Western blotting to evaluate PCSK9-induced reduction of hepatic LDLR protein (R&D Biosystems, #AF2255) using Actb (Sigma #A5441) for normalization.

Techniques: Clinical Proteomics, Western Blot, Enzyme-linked Immunosorbent Assay